SUCCESS!
Here is what I did. I made a clone copy of only the original "C" drive to another HD to test out different scenarios. I disconnected my original "C" drive and connected the clone drive in its place. When I booted it came up with an error about the boot sector. Windows told me to insert my Windows 7 install disk. I then rebooted from the DVD, windows started up and I selected "Repair my computer" option. The DVD ran and fixed the problem. I then rebooted and windows started up like normal. Now the new drive is a clone of "C" but still booting up from the second HD.
Next I then ran a partition resizing program. I used Macrorit Disk Partition Expert. I ran the program then selected the "C" drive from the list of drives shown in the upper window, I then clicked Resize/Move Volume. I resized the "C" drive to leave 300MB of free space. You can move the slider at the beginning or at the end of the drive image to create the new partition space, I used the beginning slider. Click on "Commit" to start the process. Follow the program prompts until finished.
When done shut down the PC, disconnect the second drive but leave only the clone drive connected. When windows tries to start up it will fail and ask you to boot from the DVD to fix the boot up issue. Again when windows started up, I selected the "Repair my computer" option. The DVD ran and it fixed the problem, then rebooted. This time windows started up like normal, and now from a single drive.